2A0K
Crystal structure of Nucleoside 2-deoxyribosyltransferase from Trypanosoma brucei at 1.8 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97980 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.004, 75.156, 87.365 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 19.800 - 1.800 |
| R-factor | 0.17466 |
| Rwork | 0.173 |
| R-free | 0.20581 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.953 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 1.897 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 32301 | |
| <I/σ(I)> | 8.6 | 2.7 |
| Completeness [%] | 94.4 | 74.22 |
| Redundancy | 4.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 30 % PEG MME 2000, 0.2 ammonium sulfate, 0.1 sodium acetate trihydrate pH 4.6 , VAPOR DIFFUSION, SITTING DROP, temperature 298K |
