2ZZS
Crystal structure of cytochrome c554 from Vibrio parahaemolyticus strain RIMD2210633
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-05 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 84.949, 87.612, 103.847 |
| Unit cell angles | 71.50, 72.98, 83.68 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.19519 |
| Rwork | 0.193 |
| R-free | 0.24213 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gdv |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.777 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.063 | 0.205 |
| Number of reflections | 223962 | |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 94.5 | 77.9 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 10mM sodium phosphate buffer (pH 7.0), 0.1M phosphate-citrate (pH 4.2), 0.2M NaCl, 20%(w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
