2ZVO
NEMO CoZi domain in complex with diubiquitin in C2 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.861, 98.372, 50.616 |
| Unit cell angles | 90.00, 101.96, 90.00 |
Refinement procedure
| Resolution | 49.200 - 2.900 |
| R-factor | 0.23956 |
| Rwork | 0.237 |
| R-free | 0.27699 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.282 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
| Rmerge | 0.162 | 0.097 | 0.236 |
| Number of reflections | 15653 | ||
| <I/σ(I)> | 6.462 | ||
| Completeness [%] | 88.9 | 99.8 | 53 |
| Redundancy | 2.9 | 3.5 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | VAPOR DIFFUSION, SITTING DROP |
