2ZSW
Crystal structure of H-2Kb in complex with the Q600Y variant of JHMV epitope S598
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10-29 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.711, 90.147, 91.969 |
| Unit cell angles | 81.11, 70.58, 68.25 |
Refinement procedure
| Resolution | 27.000 - 2.800 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.25284 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g7q |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 2.910 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.101 | 0.303 |
| Number of reflections | 44395 | |
| <I/σ(I)> | 10 | 2.1 |
| Completeness [%] | 95.8 | 85.6 |
| Redundancy | 2.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1M cacodylate, 13% PEG 8K, 0.2M Ca(OAc)2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
