2ZQQ
Crystal structure of human AUH (3-methylglutaconyl-coa hydratase) mixed with (AUUU)24A RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-23 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.740, 129.276, 88.586 |
| Unit cell angles | 90.00, 94.32, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hzd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.120 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.071 | 0.164 |
| Number of reflections | 88656 | |
| <I/σ(I)> | 23.6 | 4.725 |
| Completeness [%] | 92.8 | 85.5 |
| Redundancy | 2.29 | 1.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | DROP: 50MM HEPES, 1.0M AMMONIUM SULFATE, 1.0% PEG400, RESERVOIR: 100MM HEPES, 2.0M AMMONIUM SULFATE, 2.0% PEG400, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
