2ZPX
TNF Receptor Subtype One-selective TNF Mutant with Antagonistic Activity; R1antTNF-T8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-06 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.586, 111.331, 75.382 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.830 |
| R-factor | 0.268 |
| Rwork | 0.268 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tnf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.670 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.244 | 0.530 |
| Number of reflections | 10337 | |
| <I/σ(I)> | 36.4 | 3.16 |
| Completeness [%] | 92.9 | 85.6 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 15% PEG1500, 0.2M Calcium Acetate, 0.1M Sodium Cacodylate, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
