2ZP1
Structual basis of iodo-tyrosine recognition by engineered archeal tyrosyl-tRNA synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-03 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 102.201, 102.201, 71.904 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.650 - 1.700 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zh0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.064 | 0.734 |
| Number of reflections | 42504 | |
| <I/σ(I)> | 43.41 | 2.75 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 13.7 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 24-28% PEG300, 5-8% PEG8000, 10% Glycerol, 0.1M TRIS, PH8.5, VAPOR DIFFUSION, TEMPERATURE 293K, pH 8.50 |
