2ZP0

Human factor viia-tissue factor complexed with benzylsulfonamide-D-ile-gln-P-aminobenzamidine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-02-29
Detector	RIGAKU
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	71.370, 81.990, 123.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.940 - 2.700
Rwork	0.218
R-free	0.30600
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1dan
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNX (2002)
Refinement software	CNX (2002)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.700
Rmerge	0.103
Number of reflections	19950
<I/σ(I)>	4.8
Completeness [%]	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	8 % PEG 5000, 0.1M sodium chloride, 0.005M calcium chloride, 0.1M cacodylate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K