2ZOL
Crystal structure of H-2Db in complex with the W513S variant of JHMV epitope S510
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 83.561, 71.033, 86.999 |
| Unit cell angles | 90.00, 103.45, 90.00 |
Refinement procedure
| Resolution | 33.700 - 2.700 |
| R-factor | 0.2564 |
| Rwork | 0.254 |
| R-free | 0.30726 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bz9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.960 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.390 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.083 | 0.330 |
| Number of reflections | 27219 | |
| <I/σ(I)> | 8.2 | 3.2 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 294 | 0.1M sodium citrate, 28% PEG 3350, 0.15M lithium sulfate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
