2ZKS
Structural insights into the proteolytic machinery of apoptosis-inducing Granzyme M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 1W2B |
| Synchrotron site | BSRF |
| Beamline | 1W2B |
| Temperature [K] | 93 |
| Detector technology | FLAT PANEL |
| Collection date | 2008-03-05 |
| Detector | MARRESEARCH Flat Panel |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 74.671, 74.671, 113.557 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.190 - 2.700 |
| R-factor | 0.22609 |
| Rwork | 0.223 |
| R-free | 0.29308 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2GZC |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.880 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 64.680 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.094 |
| Number of reflections | 9978 |
| <I/σ(I)> | 7.3 |
| Completeness [%] | 95.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 20% PEG3350, 0.2M Li2SO4, 0.1M Bicine, pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
