2ZKJ
Crystal structure of human PDK4-ADP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.616, 68.743, 81.554 |
| Unit cell angles | 90.00, 100.10, 90.00 |
Refinement procedure
| Resolution | 49.220 - 2.000 |
| R-factor | 0.18771 |
| Rwork | 0.185 |
| R-free | 0.24016 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y8n |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.831 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 | 0.665 |
| Number of reflections | 52354 | |
| <I/σ(I)> | 23.4 | 2.4 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.9M ammonium sulfate, 4% polyethylene glycol 400, 100mM potassium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
