2ZKH
Human thrombopoietin neutralizing antibody TN1 FAB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.667, 71.416, 104.433 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.140 - 2.040 |
| R-factor | 0.1951 |
| Rwork | 0.193 |
| R-free | 0.23167 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hrp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.328 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.140 | 2.120 |
| High resolution limit [Å] | 2.040 | 2.050 |
| Rmerge | 0.093 | 0.358 |
| Number of reflections | 26573 | |
| <I/σ(I)> | 10.3 | 6.6 |
| Completeness [%] | 99.0 | 97.7 |
| Redundancy | 5.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 0.2M potassium sodium tartrate, 20%W/V polyethylene glycol 3350, 19mg/ml protein concentration, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
