2ZJR

Refined native structure of the large ribosomal subunit (50S) from Deinococcus radiodurans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	I 2 2 2
Unit cell lengths	169.900, 408.900, 694.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.900 - 2.910
Rwork	0.277
R-free	0.31100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nkw
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.910	3.040
High resolution limit [Å]	2.910	2.910
Number of reflections	493787
Completeness [%]	94.1	73

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP