2ZI6

C4S dCK variant of dCK in complex with D-dA+UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-10-06
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	138.250, 138.510, 118.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.770
Rwork	0.208
R-free	0.28340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENRTY 1P5Z
RMSD bond length	0.007
RMSD bond angle	0.027
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.870
High resolution limit [Å]	1.770	1.770
Rmerge	0.071	0.414
Number of reflections	109802
<I/σ(I)>	13.4	3
Completeness [%]	98.6	93
Redundancy	4.8	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	285	1.0M Sodium Citrate, 100mM Hepes, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K