2ZET
Crystal structure of the small GTPase Rab27B complexed with the Slp homology domain of Slac2-a/melanophilin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 82.208, 82.208, 325.484 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.280 - 3.000 |
| R-factor | 0.247 |
| Rwork | 0.247 |
| R-free | 0.29600 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 23600 | |
| <I/σ(I)> | 16 | 2.45 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 1.65M (NH4)2SO4, 0.1M Tris-HCl, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
