2ZC3
Penicillin-binding protein 2X (PBP 2X) acyl-enzyme complex (biapenem) from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL32B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-16 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 106.789, 171.909, 88.666 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.510 - 2.500 |
| R-factor | 0.2369 |
| Rwork | 0.235 |
| R-free | 0.28126 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z2l |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.960 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.510 | 2.580 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.059 | 0.228 |
| Number of reflections | 53714 | |
| <I/σ(I)> | 18.9 | 5.5 |
| Completeness [%] | 92.7 | 90.1 |
| Redundancy | 5.3 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10-25% PEG4000, 0.2M ammonium sulfate, 0.1M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
