2Z7L
Unphosphorylated Mitogen Activated Protein Kinase ERK2 in Complex with (4-{[5-Carbamoyl-4-(3-Methylanilino)Pyrimidin 2-Yl]Amino}Phenyl)Acetic Acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-06 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.873, 69.962, 60.775 |
| Unit cell angles | 90.00, 109.58, 90.00 |
Refinement procedure
| Resolution | 44.310 - 2.410 |
| Rwork | 0.223 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3erk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNX (2002) |
| Refinement software | CNX (2002) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.310 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.077 |
| Number of reflections | 12389 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | PEG550 MME, ethylene glycol, beta-mercaptoethanol, pH7.0, VAPOR DIFFUSION, SITTING DROP |
