2Z64

Crystal structure of mouse TLR4 and mouse MD-2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-28
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	83.158, 101.882, 126.364
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.840
R-factor	0.24449
Rwork	0.242
R-free	0.29043
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z63
RMSD bond length	0.010
RMSD bond angle	1.421
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Number of reflections	23932
Completeness [%]	90.1
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	296	0.1M Na-Cacodylate, 23% PEG 8000, pH 6.5, VAPOR DIFFUSION, temperature 296K