2Z5Y
Crystal Structure of Human Monoamine Oxidase A (G110A) with Harmine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-12 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 135.547, 217.364, 54.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.240 - 2.170 |
| R-factor | 0.19592 |
| Rwork | 0.193 |
| R-free | 0.24358 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o5w |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.812 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.570 | 2.250 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.079 | 0.310 |
| Number of reflections | 31614 | |
| <I/σ(I)> | 7.1 | 1.5 |
| Completeness [%] | 71.4 | 21.5 |
| Redundancy | 2.8 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 1.6M AMMONIUM SULFATE, 100mM CITRIC ACID, pH 5.00, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
