2Z5X
Crystal Structure of Human Monoamine Oxidase A with Harmine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-16 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 135.263, 218.706, 54.373 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.540 - 2.200 |
| R-factor | 0.20408 |
| Rwork | 0.201 |
| R-free | 0.25531 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o5w |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.125 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.280 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.138 | 0.670 |
| Number of reflections | 41775 | |
| <I/σ(I)> | 6.7 | 1.6 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 1.6M AMMONIUM SULFATE, 100mM CITRIC ACID, pH 5.60, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
