2Z2W
Human Wee1 kinase complexed with inhibitor PF0335770
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-25 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 69.747, 69.747, 157.215 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.920 - 2.220 |
| R-factor | 0.21795 |
| Rwork | 0.215 |
| R-free | 0.27064 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x8b |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.433 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.075 | 0.530 |
| Number of reflections | 19968 | |
| <I/σ(I)> | 46 | 3.5 |
| Completeness [%] | 96.8 | 68.9 |
| Redundancy | 12.8 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | NaCL, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
