2YZ1
Crystal structure of the ligand-binding domain of murine SHPS-1/SIRP alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-03 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.555, 57.670, 69.186 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.750 - 1.400 |
| R-factor | 0.17553 |
| Rwork | 0.174 |
| R-free | 0.19914 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.040 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.750 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.055 | 0.340 |
| Number of reflections | 39022 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 32% PEG 4000, 0.1M PIPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
