2YRF

Crystal structure of 5-methylthioribose 1-phosphate isomerase from Bacillus subtilis complexed with sulfate ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2004-09-27
Detector	RIGAKU RAXIS V
Wavelength(s)	1.0
Spacegroup name	P 41
Unit cell lengths	69.273, 69.273, 154.436
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.360 - 2.700
R-factor	0.225
Rwork	0.225
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w2w
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	69.200
High resolution limit [Å]	2.700
Number of reflections	18662

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	50mM MES, 50mM Tris (pH7.4), 1.55M ammonium sulfate, 5% 1,4-dioxane, 6% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K