2YPR
Crystal structure of the DNA binding ETS domain of human protein FEV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-26 |
Detector | ADSC CCD |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 130.770, 130.770, 130.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 75.500 - 2.640 |
R-factor | 0.2162 |
Rwork | 0.216 |
R-free | 0.22720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fli |
RMSD bond length | 0.008 |
RMSD bond angle | 0.880 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.500 | 2.770 |
High resolution limit [Å] | 2.640 | 2.640 |
Rmerge | 0.090 | |
Number of reflections | 11808 | |
<I/σ(I)> | 19.4 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 15.9 | 16.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 14% PEG3350, 5% GLYCEROL, 100 MM CITRATE PH 5.3 |