2YPO
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase with phenylalanine bound in only one site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 203.969, 203.969, 66.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.160 - 2.000 |
| R-factor | 0.13932 |
| Rwork | 0.138 |
| R-free | 0.15930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nv8 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.002 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.160 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 0.470 |
| Number of reflections | 106936 | |
| <I/σ(I)> | 11.5 | 1.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M TRIS-HCL PH 7.5, 1.5 M AMMONIUM SULFATE, 12% V/V GLYCEROL |
