2YPN

Hydroxymethylbilane synthase

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID09
Synchrotron site	ESRF
Beamline	ID09
Temperature [K]	293
Detector technology	CCD
Collection date	1995-11-15
Wavelength(s)	0.4,1.6
Spacegroup name	P 21 21 2
Unit cell lengths	88.060, 75.730, 50.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.500 - 2.300
Rwork	0.194
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ah5
RMSD bond length	0.007
RMSD bond angle	1.574
Data reduction software	LAUENORM/AGROVATA
Data scaling software	LAUENORM
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1F)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.500	2.390
High resolution limit [Å]	2.300	2.300
Rmerge	0.102	0.258
Number of reflections	13949
<I/σ(I)>	5.9	2.4
Completeness [%]	89.8	89.6
Redundancy	11	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.3		PROTEIN WAS CRYSTALLISED AT PH 5.3 IN ITTING DROPS OF 0.05ML WITH 6-7MG/ML OF PROTEIN, 0.3MM EDTA, 15MM DITHIOTHREITOL, 10%(W/V) PEG6000 AND 0.01% NAN3 IN 0.1M NAAC.