2YOZ
Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, crystallized with 2'-AMPS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-04-03 |
Detector | MAR555 FLAT PANEL |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.400, 47.880, 51.150 |
Unit cell angles | 90.00, 94.45, 90.00 |
Refinement procedure
Resolution | 16.999 - 2.100 |
R-factor | 0.1773 |
Rwork | 0.175 |
R-free | 0.22220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xmi |
RMSD bond length | 0.004 |
RMSD bond angle | 0.817 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.100 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.080 | 0.520 |
Number of reflections | 10896 | |
<I/σ(I)> | 11.7 | 2 |
Completeness [%] | 99.4 | 98.6 |
Redundancy | 3.5 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4 | 277 | 250 UM PROTEIN AND 10 MM 2, 3(SP)-CYCLIC-AMPS MIXED IN 0.5 PLUS 0.5 DROPS WITH 30% PEG4000 AND 50 MM ACETATE (1:1 MIX OF PH 3 AND PH 5) IN 4C TEMP |