2YOB
High resolution AGXT_M structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.495, 103.525, 153.832 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.950 - 1.900 |
| R-factor | 0.1713 |
| Rwork | 0.170 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h0c |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.064 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.220 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.910 |
| Rmerge | 0.100 | 0.540 |
| Number of reflections | 69440 | |
| <I/σ(I)> | 20.2 | 1.4 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 8.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 15% PEG 3350, 0.1M BIS-TRIS PH 5.2, 150MM LI2SO4 AND 5% W/V OCTYL-B-D-GLUCOSIDE |
