2YL5
Inhibition of the pneumococcal virulence factor StrH and molecular insights into N-glycan recognition and hydrolysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.250, 115.860, 132.260 |
| Unit cell angles | 90.00, 99.62, 90.00 |
Refinement procedure
| Resolution | 38.250 - 2.150 |
| R-factor | 0.23979 |
| Rwork | 0.237 |
| R-free | 0.29282 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.802 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.630 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.140 | 0.380 |
| Number of reflections | 105278 | |
| <I/σ(I)> | 10.4 | 3.5 |
| Completeness [%] | 97.1 | 93 |
| Redundancy | 4.8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
