2YK4
Structure of Neisseria LOS-specific sialyltransferase (NST).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 86.565, 124.735, 41.866 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.070 - 1.940 |
R-factor | 0.19639 |
Rwork | 0.195 |
R-free | 0.22274 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yk5 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.619 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.070 | 1.970 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.010 | 0.050 |
Number of reflections | 34365 | |
<I/σ(I)> | 35.5 | 3.22 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 8.8 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.4 | PROTEIN WAS CRYSTALLIZED BY VAPOR DIFFUSION METHODS USING DROPS OF PROTEIN MIXED WITH AN EQUAL VOLUME OF PRECIPITANT: 100 MM SODIUM ACETATE (PH 4.2 - 4.4) 1.7 M DI-AMMONIUM SULFATE. |