2YHM
Structure of respiratory syncytial virus nucleocapsid protein, P212121 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 133.607, 149.914, 255.196 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.600 |
| R-factor | 0.19778 |
| Rwork | 0.196 |
| R-free | 0.22359 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wj8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.091 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.730 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.140 | 0.860 |
| Number of reflections | 53027 | |
| <I/σ(I)> | 14.1 | 2.5 |
| Completeness [%] | 81.7 | 66.2 |
| Redundancy | 11.2 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 10% (W/V) PEG 400, 0.1 M KCL, 0.05 M HEPES PH 7.0, 0.01 M CACL2 |
