2YG9
Structure of an unusual 3-Methyladenine DNA Glycosylase II (Alka) from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.311, 51.372, 90.039 |
| Unit cell angles | 90.00, 99.71, 90.00 |
Refinement procedure
| Resolution | 42.680 - 1.950 |
| R-factor | 0.17315 |
| Rwork | 0.170 |
| R-free | 0.23101 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yg8 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.476 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.680 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.140 | 0.480 |
| Number of reflections | 35790 | |
| <I/σ(I)> | 8.8 | 3 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1 UL DROPS OF 10 MG/ML PROTEIN MIXED WITH A SOLUTION CONTAINING 1 M LICL2, 0.1 M MES PH 6.0 AND 10% PEG6000 |
