2YG8
Structure of an unusual 3-Methyladenine DNA Glycosylase II (Alka) from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.562, 51.297, 90.458 |
| Unit cell angles | 90.00, 99.76, 90.00 |
Refinement procedure
| Resolution | 49.750 - 2.000 |
| R-factor | 0.16054 |
| Rwork | 0.158 |
| R-free | 0.20315 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.430 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.750 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.120 | 0.390 |
| Number of reflections | 33423 | |
| <I/σ(I)> | 14.8 | 5.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.3 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1 UL DROPS OF 10 MG/ML PROTEIN MIXED WITH A SOLUTION CONTAINING 1 M LICL2, 0.1 M MES PH 6.0 AND 10% PEG6000 |
