2YDQ
CpOGA D298N in complex with hOGA-derived O-GlcNAc peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-22 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 61 |
| Unit cell lengths | 118.210, 118.210, 148.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.700 - 2.600 |
| R-factor | 0.19376 |
| Rwork | 0.193 |
| R-free | 0.23053 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.365 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.160 |
| Number of reflections | 36085 |
| <I/σ(I)> | 36.9 |
| Completeness [%] | 100.0 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.6M NAAC, 0.175M CDSO4, 0.1M HEPES PH 7.5 |
