2YDP
Structure of the E242A mutant of the alpha-l-arabinofuranosidase arb93a from fusarium graminearum in complex with an iminosugar inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.930, 57.800, 91.600 |
| Unit cell angles | 90.14, 93.55, 114.50 |
Refinement procedure
| Resolution | 33.970 - 1.850 |
| R-factor | 0.174 |
| Rwork | 0.171 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w5n |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.528 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.470 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.100 | 0.370 |
| Number of reflections | 86184 | |
| <I/σ(I)> | 5.5 | 1.8 |
| Completeness [%] | 95.2 | 95.2 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 20% PEG 8000, 100 MM SODIUM CACODYLATE PH 6.5, 200 MM LISO4, 20% GLYCEROL AS CYOPROTECTANT. |
