2YDO
Thermostabilised HUMAN A2a Receptor with adenosine bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-09 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.465, 98.869, 79.516 |
| Unit cell angles | 90.00, 93.49, 90.00 |
Refinement procedure
| Resolution | 60.420 - 3.000 |
| R-factor | 0.24562 |
| Rwork | 0.244 |
| R-free | 0.26896 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eml |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.983 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0100) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.340 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.100 | 0.550 |
| Number of reflections | 10556 | |
| <I/σ(I)> | 7.1 | 2.2 |
| Completeness [%] | 93.9 | 95 |
| Redundancy | 2.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 0.05 M TRISHCL, PH 7.6, 9.6% PEG 200, 22.9%. PEG 300 |
