2YD0
Crystal structure of the soluble domain of human endoplasmic reticulum aminopeptidase 1 ERAP1
Replaces: 2XDTExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 200.948, 200.948, 114.222 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.940 - 2.700 |
| R-factor | 0.15496 |
| Rwork | 0.152 |
| R-free | 0.21529 |
| Structure solution method | MIR |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.668 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.200 | 0.960 |
| Number of reflections | 37682 | |
| <I/σ(I)> | 12.3 | 3.2 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 12.2 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.4M KCITRATE, 100 MM CACODYLATE (PH 5.7), 170 MM N-DODECYL-BETA-MALTOSIDE |
