2YCZ
TURKEY BETA1 ADRENERGIC RECEPTOR WITH STABILISING MUTATIONS AND BOUND ANTAGONIST IODOCYANOPINDOLOL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-14 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.855, 56.298, 110.939 |
| Unit cell angles | 90.00, 116.39, 90.00 |
Refinement procedure
| Resolution | 41.310 - 3.650 |
| R-factor | 0.25005 |
| Rwork | 0.249 |
| R-free | 0.27046 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vt4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.925 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.300 | 3.710 |
| High resolution limit [Å] | 3.650 | 3.650 |
| Rmerge | 0.100 | 0.510 |
| Number of reflections | 11215 | |
| <I/σ(I)> | 9.6 | 3.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.0 |
