2YCX
TURKEY BETA1 ADRENERGIC RECEPTOR WITH STABILISING MUTATIONS AND BOUND ANTAGONIST CYANOPINDOLOL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-19 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.029, 56.434, 111.410 |
| Unit cell angles | 90.00, 118.33, 90.00 |
Refinement procedure
| Resolution | 39.500 - 3.250 |
| R-factor | 0.27365 |
| Rwork | 0.271 |
| R-free | 0.32465 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vt4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.290 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.500 | 3.340 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.140 | 0.490 |
| Number of reflections | 11157 | |
| <I/σ(I)> | 8.3 | 2 |
| Completeness [%] | 85.0 | 41 |
| Redundancy | 4.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.3 | pH 7.3 |
