2YBV
STRUCTURE OF RUBISCO FROM THERMOSYNECHOCOCCUS ELONGATUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 115.480, 163.470, 163.540 |
| Unit cell angles | 90.00, 108.96, 90.00 |
Refinement procedure
| Resolution | 39.840 - 2.300 |
| R-factor | 0.19763 |
| Rwork | 0.196 |
| R-free | 0.23167 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rub |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.782 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.170 | 0.430 |
| Number of reflections | 253864 | |
| <I/σ(I)> | 11.11 | 3.8 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 5% PEG8000, 20% PEG200, 10% GLYCEROL, 100MM HEPES, PH 7 |
