2YBS

JMJD2A COMPLEXED WITH S-2-HYDROXYGLUTARATE AND HISTONE H3K36me3 PEPTIDE (30-41)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-26
Detector	RIGAKU SATURN 944
Spacegroup name	P 21 21 2
Unit cell lengths	100.924, 149.354, 57.271
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.140 - 2.320
R-factor	0.214
Rwork	0.214
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ox0
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.140	2.400
High resolution limit [Å]	2.320	2.320
Rmerge	0.070	0.360
Number of reflections	36569
<I/σ(I)>	19.69	4.26
Completeness [%]	94.9	97.2
Redundancy	5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.1 M CITRATE PH 5.5, 4 MM NICL2, 20% PEG 3350, VAPOUR DIFFUSION, SITTING DROP, TEMPERATURE 277K.