2YAZ
The Crystal Structure of Leishmania major dUTPase in complex dUMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 109.110, 109.110, 309.311 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 103.100 - 2.400 |
| R-factor | 0.20383 |
| Rwork | 0.203 |
| R-free | 0.22532 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cje |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.314 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.600 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.120 | 0.910 |
| Number of reflections | 84915 | |
| <I/σ(I)> | 16.5 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.1 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M NACL, 0.1 M BIS-TRIS PH 6.5, 1.5 M (NH4)2 SO4. |
