2Y85
CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS PHOSPHORIBOSYL ISOMERASE WITH BOUND RCDRP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.980, 121.070, 81.140 |
| Unit cell angles | 90.00, 95.60, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.21663 |
| Rwork | 0.213 |
| R-free | 0.28726 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vzw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.234 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.160 | 0.600 |
| Number of reflections | 34306 | |
| <I/σ(I)> | 4.9 | 2.1 |
| Completeness [%] | 99.3 | 95.1 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.7 | 32% PEG 6000, 0.1M SODIUM CITRATE PH 5.7, 5MM RCDRP. |
