2Y7S
Structure of a designed meningococcal antigen (factor H binding protein, mutant G1) inducing broad protective immunity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-04 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.480, 99.650, 58.060 |
| Unit cell angles | 90.00, 97.82, 90.00 |
Refinement procedure
| Resolution | 49.817 - 1.900 |
| R-factor | 0.1879 |
| Rwork | 0.186 |
| R-free | 0.23270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w80 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.117 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.370 |
| Number of reflections | 41959 | |
| <I/σ(I)> | 9.4 | 3.5 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | 20% PEG 3350, 0.2M AMMONIUM FORMATE, PH 6.6 |
