2Y7Q

THE HIGH-AFFINITY COMPLEX BETWEEN IGE AND ITS RECEPTOR FC EPSILON RI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	99.491, 103.341, 110.122
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.680 - 3.400
R-factor	0.248
Rwork	0.245
R-free	0.29200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1F6A AND 1O0V
RMSD bond length	0.006
RMSD bond angle	1.104
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.000	3.490
High resolution limit [Å]	3.400	3.400
Rmerge	0.250
Number of reflections	16204
<I/σ(I)>	8.5	1.6
Completeness [%]	99.9	99.9
Redundancy	5.9	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7		SITTING DROP VAPOR DIFFUSION. RESERVOIR SOLUTION CONTAINED 2.8M SODIUM ACETATE PH 7.