2Y6R
Structure of the TetX monooxygenase in complex with the substrate 7- chlortetracycline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-25 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.416, 78.870, 86.609 |
| Unit cell angles | 110.99, 90.27, 92.87 |
Refinement procedure
| Resolution | 48.330 - 3.100 |
| R-factor | 0.22672 |
| Rwork | 0.225 |
| R-free | 0.25616 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xdo |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.439 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.330 | 3.210 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.110 | 0.430 |
| Number of reflections | 29712 | |
| <I/σ(I)> | 9.6 | 2.1 |
| Completeness [%] | 96.2 | 92.5 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
