2Y6O
Crystal structure of EphA4 kinase domain in complex with Dasatinib.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-07-01 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.412, 91.636, 98.346 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.588 - 1.543 |
| R-factor | 0.1811 |
| Rwork | 0.180 |
| R-free | 0.20050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y6m |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.994 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.560 | 1.630 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.070 | 0.320 |
| Number of reflections | 41896 | |
| <I/σ(I)> | 14.8 | 4.5 |
| Completeness [%] | 95.4 | 82.8 |
| Redundancy | 4.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | AMMONIUM SULFATE, BIS TRIS PH 5.5, PEG 10K |
