2Y52
Crystal structure of E496A mutant of the box pathway encoded ALDH from Burkholderia xenovorans LB400
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.050, 68.130, 78.080 |
| Unit cell angles | 112.23, 90.56, 111.69 |
Refinement procedure
| Resolution | 38.080 - 1.650 |
| R-factor | 0.18298 |
| Rwork | 0.180 |
| R-free | 0.23107 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.002 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.080 |
| High resolution limit [Å] | 1.650 |
| Rmerge | 0.090 |
| Number of reflections | 117024 |
| <I/σ(I)> | 7.2 |
| Completeness [%] | 95.9 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
