2Y3V

N-terminal head domain of Danio rerio SAS-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	77
Detector technology	CCD
Collection date	2009-12-12
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	66.990, 95.270, 129.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.799 - 1.920
R-factor	0.227
Rwork	0.226
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SE-MET DERIVATIVE
RMSD bond length	0.002
RMSD bond angle	0.663
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.800	2.020
High resolution limit [Å]	1.920	1.920
Rmerge	0.130	1.500
Number of reflections	63745
<I/σ(I)>	12.4	1.8
Completeness [%]	99.4	98.6
Redundancy	12.3	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		0.1 M TRIS PH 8.5, 25% PEG 3350.