2Y3Q

1.55A structure of apo bacterioferritin from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 42 21 2
Unit cell lengths	208.000, 208.000, 142.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.230 - 1.550
R-factor	0.16652
Rwork	0.165
R-free	0.20007
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vxi
RMSD bond length	0.014
RMSD bond angle	1.649
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.200	1.610
High resolution limit [Å]	1.550	1.550
Rmerge	0.100	0.620
Number of reflections	438427
<I/σ(I)>	12.8	3
Completeness [%]	98.4	99.2
Redundancy	4.1	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		1.8M AMMONIUM SULPHATE, 100MM ACETATE, PH 5.5